diff --git a/src/boot_verifier.py b/src/boot_verifier.py
index 23446d9..10c2ec3 100644
--- a/src/boot_verifier.py
+++ b/src/boot_verifier.py
@@ -124,6 +124,100 @@ def verify_boot(
     )
 
 
+def verify_zynq_status(
+    config: Config,
+    timeout: int | None = None,
+    max_retries: int = 3,
+    uart_available: bool = True,
+    callback: ProgressCallback = None,
+) -> BootVerificationResult:
+    """Quickly verify Zynq is responding after a download operation.
+
+    Performs a fast ping check and optionally a serial port read.
+    Unlike verify_boot, this is a lightweight check meant to confirm
+    the device is alive after flash/TFTP operations (before reboot).
+
+    Args:
+        config: Application configuration.
+        timeout: Seconds per ping attempt.
+        max_retries: Number of ping retry attempts.
+        uart_available: Whether UART serial port is available.
+        callback: Optional progress callback.
+
+    Returns:
+        BootVerificationResult (ip_reachable and serial_found reflect
+        the quick check results).
+    """
+    timeout = timeout or config.ping_timeout
+    ip = config.zynq_ip
+
+    if callback:
+        callback("start", f"Checking Zynq status on {ip}...")
+
+    # Check 1: Ping
+    ip_reachable = False
+    for attempt in range(1, max_retries + 1):
+        if callback:
+            callback("progress", f"Ping attempt {attempt}/{max_retries}...")
+        if ping_ip(ip, 1, timeout):
+            ip_reachable = True
+            if callback:
+                callback("complete", f"Device {ip} is reachable")
+            break
+        time.sleep(1)
+
+    # Check 2: Serial (only if UART is available)
+    serial_found = False
+    boot_info: dict = {}
+    if uart_available and config.serial_port:
+        ports = detect_serial_ports()
+        if ports:
+            for port_info in ports:
+                if port_info.device == config.serial_port:
+                    try:
+                        lines = read_serial_stream(
+                            config.serial_port,
+                            config.serial_baudrate,
+                            timeout=3,
+                        )
+                        parsed = parse_boot_output(lines)
+                        if parsed.ip_address or parsed.boot_message or parsed.version:
+                            serial_found = True
+                            boot_info = {
+                                "ip_address": parsed.ip_address,
+                                "version": parsed.version,
+                                "boot_message": parsed.boot_message,
+                                "raw_lines": lines[-20:],
+                            }
+                            if callback:
+                                callback("complete", "Boot messages detected on serial")
+                            break
+                    except Exception:
+                        pass  # Non-fatal; fall through
+
+    success = ip_reachable or serial_found
+    message_parts = []
+    if ip_reachable:
+        message_parts.append(f"IP {ip} reachable")
+    if serial_found:
+        message_parts.append("serial boot info found")
+    if not success:
+        message_parts.append("Zynq not responding")
+
+    message = "; ".join(message_parts) if message_parts else "Check inconclusive"
+
+    if callback:
+        callback("complete" if success else "error", message)
+
+    return BootVerificationResult(
+        success=success,
+        message=message,
+        ip_reachable=ip_reachable,
+        serial_found=serial_found,
+        boot_info=boot_info if boot_info else {},
+    )
+
+
 def wait_for_boot(
     config: Config,
     timeout: int | None = None,
diff --git a/src/config_manager.py b/src/config_manager.py
index 73941cd..ce030ba 100644
--- a/src/config_manager.py
+++ b/src/config_manager.py
@@ -27,6 +27,8 @@ DEFAULT_CONFIG: dict[str, Any] = {
     "reboot_timeout": 30,
     "ping_timeout": 5,
     "ping_count": 3,
+    "uart_delay": 3,
+    "inter_step_delay": 2,
 }
 
 
@@ -50,6 +52,8 @@ class Config:
     reboot_timeout: int = 30
     ping_timeout: int = 5
     ping_count: int = 3
+    uart_delay: int = 3
+    inter_step_delay: int = 2
 
     # Internal: path to the config file on disk
     _config_path: Path = field(default=None, init=False, repr=False)
@@ -151,6 +155,8 @@ class Config:
             "reboot_timeout": self.reboot_timeout,
             "ping_timeout": self.ping_timeout,
             "ping_count": self.ping_count,
+            "uart_delay": self.uart_delay,
+            "inter_step_delay": self.inter_step_delay,
         }
         return data
 
diff --git a/src/gui/main_window.py b/src/gui/main_window.py
index ac5e18c..03a8c26 100644
--- a/src/gui/main_window.py
+++ b/src/gui/main_window.py
@@ -21,8 +21,8 @@ from bitstream_programmer import full_bitstream_program, BitstreamResult
 from ip_verifier import ping_ip
 from tftp_manager import tftp_upload_verify, TftpResult
 from reboot_manager import reboot_zynq, RebootResult
-from boot_verifier import verify_boot, BootVerificationResult
-from serial_monitor import detect_serial_ports, parse_boot_output
+from boot_verifier import verify_boot, verify_zynq_status, BootVerificationResult
+from serial_monitor import detect_serial_ports, parse_boot_output, check_uart_available
 from gui.styles import (
     WINDOW_WIDTH,
     WINDOW_HEIGHT,
@@ -73,6 +73,8 @@ class MainWindow(ctk.CTk):
         self._step_statuses: dict[int, str] = {}
         self._is_running = False
         self._worker_thread: threading.Thread | None = None
+        self._uart_available: bool = False
+        self._uart_message: str = ""
 
         self._load_config()
         self._build_ui()
@@ -471,7 +473,7 @@ class MainWindow(ctk.CTk):
     # ── Step Implementations ───────────────────────────────────
 
     def _run_step_1_check_environment(self) -> bool:
-        """Step 1: Check environment (Vitis/Vivado tools + Zynq IP)."""
+        """Step 1: Check environment (Vitis/Vivado tools + Zynq IP + UART)."""
         self._log_message("Step 1: Checking environment...")
 
         if not self._config:
@@ -507,8 +509,35 @@ class MainWindow(ctk.CTk):
             self._ip_status.configure(text=f"IP: {ip} ✗", text_color=DANGER_COLOR)
             return False
 
+        # Check UART availability
+        self._log_message("  Checking UART serial port...")
+        port = self._config.serial_port
+        if port:
+            available, reason = check_uart_available(port, self._config.serial_baudrate)
+            self._uart_available = available
+            self._uart_message = reason
+            if available:
+                self._log_message(f"  ✓ UART available: {reason}")
+            else:
+                self._log_message(f"  ✗ UART unavailable: {reason}")
+                self._show_uart_notification(reason)
+        else:
+            self._uart_available = False
+            self._uart_message = "No serial port configured"
+            self._log_message("  ✗ No serial port configured")
+            self._show_uart_notification(self._uart_message)
+
         return True
 
+    def _show_uart_notification(self, reason: str) -> None:
+        """Show a non-blocking UART unavailable notification in the status panel.
+
+        Args:
+            reason: Why UART is unavailable.
+        """
+        msg = f"UART 不可用 ({reason})，日志分析及检查将禁用"
+        self.after(0, lambda: self._status.set_status(msg, "warning"))
+
     def _run_step_2_program_flash(self) -> bool:
         """Step 2: Program and verify Flash."""
         if not self._config:
@@ -606,8 +635,21 @@ class MainWindow(ctk.CTk):
             self._log_message("  Skipping reboot & boot verify (TFTP failed)")
             return False
 
-        # 4b: Reboot
-        self._log_message("Step 4b: Rebooting Zynq...")
+        # 4a2: Post-download Zynq status check
+        self._log_message("Step 4b: Checking Zynq status after download...")
+        try:
+            zynq_status = verify_zynq_status(
+                self._config,
+                uart_available=self._uart_available,
+                callback=self._zynq_status_callback,
+            )
+            status = "✓" if zynq_status.success else "✗"
+            self._log_message(f"  {status} {zynq_status.message}")
+        except Exception as e:
+            self._log_message(f"  ✗ Zynq status check error: {e}")
+
+        # 4c: Reboot
+        self._log_message("Step 4c: Rebooting Zynq...")
         try:
             reboot_result = reboot_zynq(
                 self._config,
@@ -625,8 +667,8 @@ class MainWindow(ctk.CTk):
             self._log_message("  Skipping boot verify (reboot failed)")
             return False
 
-        # 4c: Verify boot
-        self._log_message("Step 4c: Verifying boot...")
+        # 4d: Verify boot
+        self._log_message("Step 4d: Verifying boot...")
         try:
             boot_result = verify_boot(
                 self._config,
@@ -659,6 +701,10 @@ class MainWindow(ctk.CTk):
         """Callback for boot verification progress."""
         self._log_message(f"  [{status}] {message}")
 
+    def _zynq_status_callback(self, status: str, message: str) -> None:
+        """Callback for post-download Zynq status check."""
+        self._log_message(f"  [{status}] {message}")
+
     # ── Run All Steps ──────────────────────────────────────────
 
     def _update_step_dependencies(self) -> None:
@@ -781,6 +827,12 @@ class MainWindow(ctk.CTk):
             if start_index == 0 and not success:
                 break
 
+            # Inter-step delay (skip after last step)
+            if i < len(step_funcs) - 1:
+                delay = self._config.inter_step_delay if self._config else 2
+                self._log_message(f"  ⏳ Waiting {delay}s before next step...")
+                time.sleep(delay)
+
         self._progress.set_value(1.0)
         self._is_running = False
         self._run_btn.configure(state="normal", text="▶ Run All Steps")
diff --git a/src/serial_monitor.py b/src/serial_monitor.py
index 5922b71..a553ae0 100644
--- a/src/serial_monitor.py
+++ b/src/serial_monitor.py
@@ -108,6 +108,56 @@ def parse_boot_output(lines: list[str]) -> BootInfo:
     return info
 
 
+def check_uart_available(
+    port: str,
+    baudrate: int = 115200,
+    timeout: float = 3.0,
+) -> tuple[bool, str]:
+    """Check whether the UART serial port is available and readable.
+
+    Attempts to open the port, clear buffers, and read a few lines.
+    If the port opens but returns no data within the timeout, it is
+    considered available (the device may simply not be outputting yet).
+    If the port cannot be opened at all, it is unavailable.
+
+    Args:
+        port: Serial port device path (e.g., '/dev/ttyUSB0').
+        baudrate: Baud rate for serial communication.
+        timeout: Seconds to wait for data after opening.
+
+    Returns:
+        Tuple of (is_available, reason_string).
+        - (True,  "Port open") — port is usable
+        - (True,  "Port open, no data yet") — port open, no data currently
+        - (False, "No serial ports detected") — system has no serial ports
+        - (False, "Port not in detected ports") — configured port missing
+        - (False, "Failed to open: <error>") — port open error
+    """
+    # 1. Quick port list check
+    ports = detect_serial_ports()
+    if not ports:
+        return False, "No serial ports detected"
+
+    # 2. Verify the configured port is among detected ones
+    if port and port not in {p.device for p in ports}:
+        return False, f"Port {port} not in detected ports"
+
+    # 3. Try to open and read
+    try:
+        with serial.Serial(port, baudrate, timeout=timeout) as ser:
+            ser.reset_input_buffer()
+            lines: list[str] = []
+            while ser.in_waiting:
+                line = ser.readline().decode("utf-8", errors="replace").strip()
+                if line:
+                    lines.append(line)
+        if lines:
+            return True, "Port open"
+        return True, "Port open, no data yet"
+    except serial.SerialException as e:
+        return False, f"Failed to open: {e}"
+
+
 def read_serial_stream(
     port: str,
     baudrate: int = 115200,